The ultimate objectives of this work were to find new potential sources of natural antioxidants agents in the food industry. This study is the first to investigate secondary metabolites and biological activities of barks and leaves of Fraxinus angustifolia Vahl. subsp. angustifolia (Oleaceae), Tunisian native tree, from two provenances (Béja and Nefza) were investigated using two solvents extracts (ethanol and distilled water).